Wikipedia Wandering :Clostridium perfingens, Gas Gangrene, Envenomation, Toxicology, Galleria mellonella

For the next period of time I’ll be posting what Wikipedia page I have found most interesting and whatever hole it lead me down on the website. This is fun because I’ll be able to demonstrate a way of tracking how one can think.

I’m sure it will only be after Having a certain number of posts that some pattern will be apparent, however how to analyze that will have to be ad-hoc (all in jest mostly)

Maybe it’s like a mental recipe book for whatever ideas they end up crystalizing.

Today’s interest: Clostridium perfingens

  1. START—> https://en.wikipedia.org/wiki/Clostridium_perfringens

  2. NEXT—> https://en.wikipedia.org/wiki/Gas_gangrene

  3. NEXT—> https://en.wikipedia.org/wiki/Envenomation

  4. NEXT—> https://en.wikipedia.org/wiki/Toxicology

  5. NEXT—> https://en.wikipedia.org/wiki/Galleria_mellonella

  6. FINISH/BONUSES—>

    1. https://en.wikipedia.org/wiki/Pestalotiopsis_microspora

    2. https://en.wikipedia.org/wiki/Ideonella_sakaiensis

    3. https://en.wikipedia.org/wiki/Bloodstream_infection

Wikipedia week 1 day 1: Citric Acid

For the next period of time I’ll be posting what Wikipedia page I have found most interesting and whatever hole it lead me down on the website. This is fun because I’ll be able to demonstrate a way of tracking how one can think.

I’m sure it will only be after Having a certain number of posts that some pattern will be apparent, however how to analyze that will have to be ad-hoc (all in jest mostly)

Maybe it’s like a mental recipe book for whatever ideas they end up crystalizing.

Today’s interest: Citric acid

I love the taste of citrus fruit and find the color of them to be lovely.

  1. Start https://en.wikipedia.org/wiki/Citric_acid?wprov=sfti1#Cosmetics,_pharmaceuticals,_dietary_supplements,_and_foods

  2. Next: https://en.wikipedia.org/wiki/Penicillium?wprov=sfti1

  3. Next: https://en.wikipedia.org/wiki/Heroin?wprov=sfti1

  4. Next https://en.wikipedia.org/wiki/Lanthanide?wprov=sfti1#

  5. Next

    https://en.wikipedia.org/wiki/Acid_dye?wprov=sfti1#

    if nothing else I hope you enjoyed the links and whatever might be interesting!

Wikipedia week 1 day 1: Citric Acid

For the next period of time I’ll be posting what Wikipedia page I have found most interesting and whatever hole it lead me down on the website. This is fun because I’ll be able to demonstrate a way of tracking how one can think.

I’m sure it will only be after Having a certain number of posts that some pattern will be apparent, however how to analyze that will have to be ad-hoc (all in jest mostly)

Maybe it’s like a mental recipe book for whatever ideas they end up crystalizing.

Today’s interest: Citric acid

I love the taste of citrus fruit and find the color of them to be lovely.

  1. Start https://en.wikipedia.org/wiki/Citric_acid?wprov=sfti1#Cosmetics,_pharmaceuticals,_dietary_supplements,_and_foods

  2. Next: https://en.wikipedia.org/wiki/Penicillium?wprov=sfti1

  3. Next: https://en.wikipedia.org/wiki/Heroin?wprov=sfti1

  4. Next https://en.wikipedia.org/wiki/Lanthanide?wprov=sfti1#

  5. Next

    https://en.wikipedia.org/wiki/Acid_dye?wprov=sfti1#

    if nothing else I hope you enjoyed the links and whatever might be interesting!

" target="_blank">Jackie's Dissertation Guide

Guide To Writing a Philosophical Dissertation

A different link to the guide. Please use these as opposed to the below as the formatting is quite spectacular compared to the below

Dissertation guidebook 

Jacqueline M. Howells, PhD in Pathobiology 

November 3rd, 2024

Table of Contents

 (TOC, because we can’t get enough abbreviations)

Table of Contents 2

Prologue 4

Heading 1 5

Heading 2 5

Heading 3 5

Heading 4 5

Heading 5 5

Heading 6 5

Chapter 1. Getting started 7

1.1: Determine the dissertation guidelines from the graduate school 7

1.2 The Final Examination or Defense 7

1.3 Deadlines 8

Chapter 2: The Manuscript Format 9

2.1 Frontmatter: 9

2.2 Type and spacing 10

Standard typeface 10

2.3 Page numbers 11

2.4 The Formatting 11

2.5 Title Page (do not number) 12

2.6 Copyright Page (do not number) 13

2.7 The Signature Page (page number should be iii) 14

2.8 Curriculum Vitae* (iv) 14

2.9 Prefaces and Acknowledgements (v) 15

2.10 Table of Contents (vi) 15

2.11 List of tables (vii) 17

2.12 List of illustrations  (figures) (viii) 17

2.13 Communication Note (not required, just thoughts) 18

2.14 Communication and organization. 20

2.15 Graphs. 22

2.16 Dating the Dissertation 23

Chapter 3: The Manuscript 24

3.1 The Abstract 24

3.2 The Dissertation 24

3.3 Introduction/Prologue 25

1. INTRODUCTION (in our case Prologue) 25

3.2 CHAPTER I: REVIEW CHAPTER INTRODUCING FOUNDATIONS FOR DATA CHAPTER 26

3.3  CHAPTER II: DATA CHAPTER 27

3.4 CHAPTER 3: Discussion & Perspectives developed 28

3.5 BIBLIOGRAPHY 28

3.6 EPILOGUE (not required, but, being me I highly recommend it if you care deeply about your work) 29

Citation 29

Chapter 2 Figures 31

Figure 2.1 Title page example. 31

Figure 2.2 Example of a copyright page. 32

Chapter 4.  Learn how you write 33

4.1) Everyone writes incredibly differently. 33

4.2) find out where and when you write the best 33

4.3) giving yourself and others grace & accepting/giving feedback. 34

Chapter 4: software & other notes. 38

Front End Aesthetic Syntax 38

1. OVERLEAF 38

CURRENTLY ACCEPTED SOFTWARE FOR BIOMEDICAL STATISTICS AND VISUALIZATION 40

1. PRISM 40

2. MATLAB 40

3. PYTHON. 40

4. MICROSOFT TEAMS 41

Figures & Communication: 42

Biorender 42

Adobe illustrator 42

Powerpoint 43

Excel- Excel hates me but I am learning to love it 43

Citation Managers 43

4. Grammarly. (2024). Grammarly (May 26 version) [Large language model]. 45



Prologue

Writing a PhD dissertation is an incredibly ambitious adventure. Much like any intriguing or worthwhile endeavors one chooses to pursue while alive, completing a dissertation  is difficult for many reasons, but resolution of these difficulties is beneficial for many students including myself. Thus I have decided to note for me and others in the future what I have learned about my own writing process.  I possess a strange form of memory that requires a trusted document that can be can refer back to and is maintained well enough that I trust it is accurate.The  aim of this document guidebook is to aid in my goals to finish my PhD and if n possible others in the future. I will discuss here formatting rules, tips and tricks first. 



You will find below an outline which seems out of place but is the first step to formatting in both google docs and abstractly if one can parse patterns meaningful to their own needs (figure 1)   This document will contain additional parts regarding expected content for each dissertation chapter, steps I took to approach each challenge I learned from. These lessons will be expressed in an  assessment of tools for workflows to describe strategies used to approach each method.  I will describe my own success and failures using each tool, and corresponding user experiences

It is my opinion that if I am struggling this much with tool use that others are too, that sharing information, strategies, and tools, in reality is much more beneficial for each individual and the group. It is my experience that it is assumed that every student will know how to  do what is considered “basic” skills like how to use the settings in google docs. This assumption is sometimes untrue for first gen students and I hope I can convince you that the   reality is that is is easier to pass information to first generation students and allow for diverse skills to develop while learning basic somewhat arbitrary rules  which have impaired learning ability despite being easy to explain or provide tools to those who did not have this information. 

As an example of this I will outline below what the sheridan writing center taught to do first with respect to outlining and formatting  title and subsections to standardize easily across a long document.this will be used to create a table of contents later in the process. . 

Title

Heading 1

Heading 2

Heading 3 

Heading 4

Heading 5

Heading 6

Subtitle

Normal text

Find the rules regarding font for dissertation formatting  as it may indicate font size may be 10-12. Format of paragraphs, which are text blocks containing introductory sentences followed by the main point of the paragraph followed by a transition sentence at minimum. Additionally , the transition sentence should link the first sentence, the second sentence and finally the next paragraph.

The above paragraph is my setting for normal text in google docs ideally I would write these out over time and fill them in to collage them across 5 years of working in a lab, While this may seem tedious, it is essential for not losing track of 200 pages as it allows you to generated a table of contents in google docs. To set each heading click on the format button and change font type to title, headings etc and change the settings for each to your needs. To generate a table of contents go to Now that the general most base framework is established I will discuss my approach to specific tools and strategies used currently by me or tried and not used, and not tried at all.to create .

Chapter 1. Getting started

1.1: Determine the dissertation guidelines from the graduate school

The doctoral dissertation and all of the associated forms and documents related to the completion of a Ph.D. must be submitted to the Graduate School by the deadlines listed above; no extensions will be granted. See Submission of the Final Copy for important clarifications regarding the final submission process. You may find this on your school’s graduate school FAQ page. If not, email someone listed, this may take an extraordinarily unbelievable amount of time, so sooner is better than later. Save. Your. Receipts. 

1.2 The Final Examination or Defense

The final examination or defense must be scheduled by the candidate at the convenience of the readers. At least two weeks prior to the final examination or defense, candidates must provide the department manager with the appropriate dissertation defense information so the Dissertation Defense Information Form can be completed and returned to the Graduate School. Dissertation Defense 

1.3 Deadlines

As of 2024 submission for Students there are multiple deadlines for submission: examples below. CHECK YOUR DEADLINES ASAP. Recently I learned that one can use the word Due Date to mean flexible and Deadline to be inflexible. Be incredibly explicit to anyone with whom you’ve discussed these topics with and maintain documentation of anything and everything regarding this topic. 

For spring graduation: April 1st 

Fall graduation: July 1, 2014 

Chapter 2: The Manuscript Format

(the hand write! haha) 

The doctoral dissertation and all of the associated forms and documents related to the completion of a Ph.D. must be submitted to the Graduate School by the deadlines listed above; no extensions will be granted.  See graduate school website for details regarding who to submit to electronically under: Submission of the Final Copy for important clarifications regarding the final submission process. The manuscript should include the following which I will go over in more depth following: 

2.1 Frontmatter: 

Title page (do not number, but include as a “page” for pagination)

Copyright Page (do not number but include as a “page” for pagination. 

Signature Page (iii) (number with roman numerals all lowercase starting at iii due to “pagination” 

Curriculum Vitae* (iv)

Preface and Acknowledgments (v)

Table of Contents (vi)

List of Tables vii List of Illustrations (viii)

Pages with arabic numerals begin with chapter 1 and onwards. 

Should any element of the preliminary pages be longer than one page, number the pages consecutively. The preliminary pages should appear in this order but not necessarily with the page numbers shown above.  The dissertation proper (including introduction, main body of the text, illustrations, appendices, and bibliography) is numbered using Arabic numerals. The numbering begins with "1" and runs consecutively to the end.  Do not place headers on each page. Use them only as appropriate to indicate major sections of the thesis (e.g., INTRODUCTION, CHAPTER 1, BIBLIOGRAPHY). They should be centered and placed two inches from the top of the paper in uppercase type.

2.2 Type and spacing

Standard typeface

These include arial and times new roman set to print at 10-, 11-, or 12-point font are acceptable. All text should be double-spaced, except for 

block quotations- do not know what these are

Captions- assuming these are for figure legends 

Long headings- do not know what these are for beyond headings that are too long to fit within the width of the page, do not want orphan (or spaced apart) lines. 

Footnotes: I have not personally seen many footnotes but these are something one can discuss with the committee.  All these should be single-spaced with a blank line between items.

2.3 Page numbers

For the title and copyright pages do not number them, but assign numbers so that the pagination (Never heard of this word but assuming it means the “meta page number” can be found).    The next pages are preliminary pages, otherwise referred to as “the front matter” The front matter includes the CV (curriculum vitae)  Each page, including blank ones, must have a number. The number should not appear on the title page or the copyright page but, these pages are assigned numbers and are included in the pagination. 

Preliminary pages (i.e. frontmatter) are numbered with lower case roman numerals, centered at the foot of the page, three-fourths of an inch from the bottom edge. The title page counts as page i but the number does not appear on the page. The remainder of the dissertation is numbered with Arabic numerals beginning with 1.

2.4 The Formatting

Most dissertations consist of preliminary pages which are numbered using Roman numerals, and the dissertation proper which is numbered using Arabic numerals. The preliminary pages must appear in the following order:

2.5 Title Page (do not number) 

A title page example for my thesis is just a page with the title of my dissertation (please see Figure 1) (not yet completed) and includes:

First line: 

 the title in 24 sized font THE TITLE SHOULD BE IN ALL CAPITAL LETTERS. 

To do this easily in google docs go the format button in the top bar of the document. 

Select  the “text” option (it has a bolded B, if on a Windows can hold down ctl “B” to bold and unbold text, something I learned just this year! Applies to italics ctl “I”) next to it From this menu, 

select from three options: lowercase, UPPERCASE, or Title Case for highlighted text (in this case the title line). 

Second line: 

“By” in 20pt font

Third Line:

 your name in 20pt font 

Fourth line:

 Degree, (B.A., or B.S. or MSc., or BSc), institute of undergraduate studies, and year of completion (i.e. graduation). In 20 pt font 

Fifth line: 

Blank

Sixth line:

 Blank

*Note: blank space is not indicated in the formatting rules from Brown’s website, for me it was dependent on the paragraph line spacing. 1-4 lines of blank space seems to be appropriate from what I can garner.

7th line: 

Thesis

8-11th line: 

Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Pathobiology at Brown University. 

12th line: 

PROVIDENCE, RHODE ISLAND. 

13th line: 

Month of Graduation, Year of Graduation. 

Example: May, 2024.

2.6 Copyright Page (do not number)

This page is an indication that you  are the author of this thesis, and is copyrighted. Personally I had no idea what this was and am still unclear as to what it means, but  I will provide the formatting for it.  This should be the second page of the thesis manuscript please see Figure 2. The copyright symbol ( C ) will turn into © if you leave out or delete the spaces between the parenthesis and the C. Be aware that this is annoying for figure legends. I am unsure if others have a way of turning this feature off once this portion is complete but I am sure there is a way. Google will be your friend for this type of “turn off auto-formatting for copyright symbol”.  

2.7 The Signature Page (page number should be iii) 

In addition to the electronic submission, all Ph.D. candidates are required to submit their signature page to the Graduate School, which may be sent electronically. Samples are available online, by request I believe. Note that: The signature page should bear the signatures of the dissertation director and all readers. The typed names of the director and readers must appear under their signature lines. Electronic signatures are acceptable. An unsigned copy of the signature page should be uploaded to the ETD system (ETD= Electronic T? D?). Some schools provide the opportunity to upload the thesis to ProQuest. I had no idea what this was and still struggle to understand what it means. However, apparently it will be available to the public and you can efficiently print out your dissertation hard copy (which I hope to do in the near future as of today November 3rd 2024.)

2.8 Curriculum Vitae* (iv)

The curriculum vitae is a statement giving a short biography of the candidate, including institutions attended, degrees and honors, titles of publications, teaching or professional experience, and other pertinent information. Please do not include date or place of birth or phone numbers. Ask your advisor or committee how this should look given experiences are quite different across individuals and departments. 

2.9 Prefaces and Acknowledgements (v)

Should be about 1 page

2.10 Table of Contents (vi)

Generate this last when setting up a document, but once some outlining has been done it is a really good idea to set this up for a doc with writing and a doc with images/tables. Figure docs such as these should have one figure or table per page with breaks in them for drafts or if using overleaf uploaded into the “images” section of the template, upon reflection PowerPoint may also be a good way to keep your figures just make sure to keep these organized in some way as the documents slow infinitely as more rich images are added. Prior I have used an excel spreadsheet to keep track of metadata regarding data organization - mostly with columns for date of experiment, name of experiment, iteration, samples used, and result(not more than a sentence), which would correspond to three other documents: 

A folder of Raw data labeled 20XX-XX-XX) or (20240607) with no dashes whatever you prefer corresponding (example: flow data from an experiment should be labeled as such) 

An excel or spreadsheet of quantified data  labeled 20XX-XX-XX) or (20240607) with labeled experiment, for example: 20240607-a6KO-validation. This spreadsheet would include a table with samples consistently labeled a6KO-1 For the first sample, a6KO-2 etc have relevant quantifiable values for generating plots or tables, and relevant materials (for example machine used, settings(voltages for example), antibodies (clone and color and company and batch), amount used of antibody, and samples with controls on one page of the spreadsheet. The next page would be labeled analysis and include relevant analysis from other programs usually, Flowjo and matlab being examples. Finally a notes column for any divergences including errors ( you will thank yourself) or issues encountered with operations of experiment. 

A PowerPoint labeled with the same date (20XX-XX-XX) or (20240607) with no dashes whatever you prefer with corresponding  graphs generated from collected data, each notes page should include relevant writing whether that be a figure legend or notes about potential troubleshooting ideas or conceptual ideas worth noting. I like to separate by experiment specifically. I include figures and notes from other papers which I was reading at the time of experiment and label all citations needed. I go back to add to these sections as necessary.



Now with the meta document, figures document, table document, and raw data folder it will be incredibly helpful for pulling relevant information from the meta doc and communicating findings. It might  take a while to figure out how to best do this so don't panic and have fun. Note: i am super color driven when it comes to this process so i also begin by color labels for different experiments and samples. Pick a palet  and stick with it, gray or black for controls. Hashed or clear or filled. Remember all colors can and should be able to be grayscale for the most part. 

There are tools in google docs and in adobe to generate a table of contents. 

At the beginning of your document In google docs go to the tab at the top bar “Insert” select at the bottom “table of contents” This table of contents is fully editable and linked so that you can jump around in your document.  The final table of contents and page numbers should be generated in adobe at the end of the writing process or in google docs. 

2.11 List of tables (vii)

Insert a page break or section break when adding figures and tables.  For these I prefer to have them in a second document and paste them in later as the document grows this will save rendering time by a lot. It is also good practice to organize these things as they can always be deleted but harder to find. Tables should have the title at the top (not like figure legends) and should be labeled according to the chapter: table 1.1, table 2.1 for example. 

2.12 List of illustrations  (figures) (viii)

For these I prefer to have them in a second document and paste them in later as the document grows this will save rendering time by a lot. It is also good practice to organize these things as they can always be deleted but harder to find. Writing this dissertation like this from the start would have potentially given me better structure for actually doing the research and publishing. Thus to complete 2 tasks with one action, write these like you are already writing them for your thesis. A live document can be helpful. These go  at the end of each chapter. 

2.13 Communication Note (not required, just thoughts)

Communication is key and an area of improvement for me personally, so having a pipeline of operations like this is incredibly helpful for the sake of those I work with and collaborate with or just wish to discuss ideas with, or just even my own mental organization of complex jargon that's required to be known. Many can attest to this becoming more of a hindrance the longer in academia as so many rules exist for how to format genes or proteins across fields.https://podcasts.apple.com/us/podcast/night-science/id1563415749?i=1000656883905 this podcast episode from Night Science explains the emotional rollercoaster of grad school from the perspective of a systems immunobiologist discussing things like creative problem selection obsession impairing progress, how language and science and art are linked in her perspective as a systems professor at Harvard, covers feelings of despair when wanting to give up but how it's a good sign bc it's what it looks like doing science at the edge. 

Taking a break or incubation is real! It's how I finally got a 4 year long troubleshooting suppression assay to work under the instruction of a senior research associate at ucsf who demanded I take a two week break without thinking about the problem and it just worked! I couldn't believe it. It's magic. One of my biggest areas of improvement has been asking for help when I needed it for even simple things because I either didn't even know I could (never crossed my mind) or felt it would be a burden to ask. I wish I could explain this better but I suspect it's just a mannerism developed or innate.

 I remember once talking with a good friend who said “I assume no news is good news” and it dawned on me that our different manners of thinking about this concept of whether no news is good or not was being judged on an axis less helpful for assessing if someone needs help. For both, the signal is the expression of news and the output being good or bad. This is a false axis precisely because we both have a different way of thinking: one in which saying something signals for action, the other of which not saying something is a signal for action.

Neither is, in my opinion, good or bad,  in some ways that seems nonsensical to me personally now that I think about how I felt initially a bit offended or defensive. So in this sense, the portion of the communication cascade which gets perturbed for many could be resolved by establishing regularly expected news delivery of some sort and ways of expressing progress that's super operational for when times get tough and language may get hard because of tiredness or frustration or even just another email you really hoped to not have to open, so that  you can still easily send off the minimum required for your team and advisor to be able to assess will be invaluable for all. Sometimes there isn't enough time to be completely composed and sometimes science is super tedious and laborious, and unexpected (remember to take experimental expected time and multiply that by 3.5 to get a better idea of how long it will take), and can be incredibly taxing but often so rewarding in terms of richness of being able to think freely.

2.14 Communication and organization.

Inherently, clear organization which can be easily accessed and understood by both advisor and advisee organized either by the advisor if they have a pipeline set up they work under and works for you. (I now don't know if I might like my advisor’s methods of organizing super classically and simply: folders for month and date and within them daily notes and relevant attachments compared to my old meta excel sheet. I don't know which I prefer at all! But importantly  both work for me. I just wish I had realized this sooner.

 I don't know how advisors feel about potentially sharing some of their methods but it can really help to see how others organize their data and relevant modalities of collecting and storing information. It's not unlikely that everyone would learn something if this was presented akin to faculty on parade even just to validate someone's way of thinking so they can learn how to best adjust to their labs pipeline as other PIs and students may have struggled with similar communication differences.

Put simply: sharing the process of writing a thesis, essentially three “papers” of different genres: review, primary, and review/perspectives/future directions, using unique diverse roadmaps to get there will increase fitness of the group through increased traits to adapt.  These details ultimately manifest in a manuscript. It helped to see multiple ways of note taking and data organization and I have been so lucky honestly to have been mentored by so many here even if it  meant switching labs because I learned a many ways of organizing, writing techniques, how to cope with setbacks, all different and some which I was capable of and others I was not. But finding the ones I was compatible with for different aspects, for example an advisor who crafts papers using similar techniques as  myself which is very oddly low in probability seemingly given the operational flow of it, but without this validation of it being ok to learn and write this way I may have not gotten to this point. 

If folks  could share these methods more even in a seminar or teaching way maybe that could help somehow for students to know who to go to for when they need a person who can provide whatever ineffable ways of perceiving and interpreting data can help with motivation due to this validation of methods being worthwhile to pursue. Additionally I absorbed tips and tricks for how I can empty my own mind from this individual in terms of being overloaded with ideas, just from seeing how they did that for themselves in PowerPoint format, like a collage while also being a hoarder of sentences and ideas meaningful to our process for whatever reason. 

Obviously this is specific, but this is my point, it is nearly impossible to expect that one individual could fulfill that role for another let alone an entire group of students. Is it possible or even something professors would be open sharing with students? Not that they have to! But if they were open to that (not even to be an official mentor but just to demonstrate the diversity of approaching research and success in academia) I think this could be really fun for both students and faculty who wanted to participate. 

2.15 Graphs. 

Graphs may be put on cross-section pages

Programs I've used to generate graphs:

Excel. Before I understood who to email for a PRISM license which I learned from another student who told me the email of the admin who provided licenses to students(this was in 2018), I used excel. I use excel for all data tables and generating graphs is easy, for the most part. One can generate dot plots, add lines to this for a histogram, add shading, create bar and whisker plots (akin to violin plots), and many more for whatever data set you're interested in. A great first pass at data visualization is through excel regardless in my opinion. Also most graphs can be generated simply for what I've seen and the downsides are that sometimes excel formats things in a super frustrating way for me. I'm known for breaking excel a lot for no known reason so that might just be me. I am beginning to appreciate it more the longer I am in science. All graphs should be able to be greyscale so excel provides this easily and in the styles pane “quick styles” one can adjust color. Stats can also be performed but I have not ventured here as excel and I are not yet close friends. 

PRISM- made for biomed work for the most part. Ask your advisor about licensing. I cannot express how much PRISM has been essential from my entire time since being in the Bluestone lab till now how helpful this program is in teaching basic stats at the same time as generating graphs linked with tables and stats. Making graphs beautiful and adding  correct stats is perfect in this program for a grad student in my opinion. It lacks the power for some bigger data sets and doesn't have linear regression as far as I'm aware of, but it has rmANOVA. 

Matlab- for everything else there's matlab which now has a linear regression package (yay!!!)

ImageJ for imaging figures I'm not as familiar with but I know it's what people ultimately use. 

R - you can make so very many things in R, but be aware that it is so open source that many packages will never be updated, and you’ll have to go to github and sort through updated source packages that were not annotated as such in the original code. Until you get to the point where you can recognize the syntax of the language, and maybe even the context, I’d HIGHLY encourage starting with a guided coding analysis such as MATLAB (while you have it free!) or PRISM. 

2.16 Dating the Dissertation 

Because degrees are conferred three times during the calendar year, the title page and abstract of a dissertation completed at any point during the academic year must be dated on the date the degree is conferred.

Chapter 3: The Manuscript 

3.1 The Abstract

“The dissertation must be accompanied by an abstract. The abstract should, in a concise manner, present the problem of the dissertation, discuss the materials and procedure or methods used, and state the results or conclusions. Mathematical formulas, diagrams, and other illustrative materials should be avoided. The abstract should not be part of the dissertation itself nor should it be included in the table of contents. It should be headed as follows:”

Abstract of (TITLE OF DISSERTATION), by (AUTHOR'S NAME), Ph.D., Brown University, May (YEAR IN WHICH DEGREE IS TO BE AWARDED).

3.2 The Dissertation 

“The dissertation proper (including introduction, main body of the text, illustrations, appendices, and bibliography) is numbered using Arabic numerals. The numbering begins with "1" and runs consecutively to the end.”

Headings for the major sections of your manuscript (i.e. dissertation) should be at the very minimum the following They should be centered and placed two inches from the top of the paper in uppercase type.

3.3 Introduction/Prologue

INTRODUCTION (in our case Prologue)

This section for me was incredibly important, it allowed me to frame the dissertation in the same way most philosophers frame their theses in reality. Take for example, the introduction to one of my favorite philosophical texts on immunology Dr. Thomas Pradeu’s Theory of the Self linked here: https://www.google.com/books/edition/The_Limits_of_the_Self/c016CuJ8r-gC?hl=en&gbpv=1&pg=PA1&printsec=frontcover. Pradeu takes advantage of the fact that the reader will be engaging with  this text first, if the reader is truly interested in what the author is trying to convey. I find these to be absolutely essential for orienting the reader to have the capacity to understand my, or any other author’s, agenda. 

Every single human being has an agenda, whether you are aware of it or not is up to how you choose to use your freedom to think. Now that I have had several months to reflect, this is my absolute favorite part of my thesis along with the epilogue. It sums up my perspective through the eyes of an individual struggling very very hard to live in their truth, to be authentic, to have integrity. Here, I use metaphor and other rhetorical devices that I learned in AP English and AP Language from my experiences in Lowell High School (a San Francisco PUBLIC high school) to entice readers who may or may not be familiar with the historical context of my own dissertation. 

As a famous Pulitzer Prize winner Kendrick Lamar once wrote in his song Meet the Graham’s “I guess integrity is lost when the metaphors doesn’t reach you” (https://genius.com/Kendrick-lamar-meet-the-grahams-lyrics) which I am going to argue is one of the strongest lines in the hit track, as it demonstrates who can abstractly understand the current, or, to be more academically minded “Contemporary”, or, to be more culturally relevant in 2024, “Catching the vibe”, or just in plain English euphemism “reading the room”, and who lacks this ability. Those who lack it tend to not acknowledge its power. Remember that!

3.2 CHAPTER I: REVIEW CHAPTER INTRODUCING FOUNDATIONS FOR DATA CHAPTER 

This should be a review chapter that assesses all relevant information as if it were a review written after you wrote your paper to demonstrate how it answers a gap in the literature.  If I could go back to redo this process I would have used my qualifying exam and time spent reading to publish a review for chapter 1 so that I’d be established for publishing my next chapter (a data chapter) as a primary research document in years 3-4 of graduate school. 

This would have been instrumental in my training and self confidence in addition to beginning the writing process and making mistakes EARLY as opposed to rushing at the end. It is not easy if you have never or rather, haven’t in quite some time, have had an opportunity to write a paper for publication, thus many of the small but most time consuming factors could be ameliorated by going into the process of a PhD knowing that one can publish a review article first

. I am unsure if bio archives accept reviews but even just being forced to go through the process and not pay the fees associated would be helpful to use in the future. These tools I will describe and methods which worked for me would be hammered out. I would give my right hand to have been using overleaf from the beginning, and to have had at least one publication from my time in graduate school.  

3.3  CHAPTER II: DATA CHAPTER

Ideally this would be published as a first authored paper following the review covered in chapter 1.  Collecting data in years 2-4 would be helpful in updating the introduction chapter too, as more research is performed and more time is spent with the project as it develops.

This chapter looks like any research chapter you have seen. 

Abstract

Introduction

Methods

Results

Discussion

Conclusion

It should also state contributions of authors and credit for those who helped in any way. 

Example from my undergraduate thesis which is freely available through UC Berkeley, a public institution. https://nature.berkeley.edu/classes/es196/projects/2013final/HowellsJ_2013.pdf

3.4 CHAPTER 3: Discussion & Perspectives developed 

This chapter should serve as a reflection of chapter 1 with additional perspective from chapter 2 (what gap did you fill? Why is this important?) Ideally, if I could go back and repeat this process, I would view it as a perspectives paper published after the second chapter, demonstrating that by performing the research aspects including design, data collection, analysis of data, and conclusions reached regarding literature gaps and how to fill future research gaps created by your data chapter, one is now able to fully grasp the context and meaning of their work. 

Thus this would be a third and final publication under the domain of “perspectives” “opinions” or “commentary” 

See your favorite journal to determine what these look like. Nature, Science, Cell, and Frontiers (my favorite) have some examples. 

 3.5 BIBLIOGRAPHY

Citations should be centered and placed two inches from the top of the paper in uppercase type. I do not know if this must be at the end of the entire document or after each chapter. I will inquire.  One should expect to have 50-100 citations per chapter. 

3.6 EPILOGUE

(not required, but, being me I highly recommend it if you care deeply about your work)Should mirror the prologue in a similar way as the introduction mirrors discussion.  The epilogue in my dissertation as cited below set up my postdoctoral scholar work. It is the true philosophy of science I have developed based on the techniques and data collected in chapter 2, the review of accepted tools, methods, and yes, unfortunately, perspectives count under acceptable or not (whether this should be the case or not is up to  you as a new philosopher, right?). This can have figures, and be far more abstract than the prior chapters. 

Citation

Howells, Jacqueline M., "Elucidating mechanisms of murine neutrophil progenitor engraftment in bone marrow" (2024). Pathobiology Theses and Dissertations. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:49eu8uxw/




Chapter 2 Figures



Figure 2.1 Title page example. I did not end up making the May Due Date unbeknownst to me! ALWAYS double check in writing when DEADLINES ARE  

Copyright page, still wish I understood when someone edited my thesis to include an @ instead of a copyright symbol. Note: it hurt me a lot to know that someone wouldn’t take the time to explain this to me nor their edits. it sucked my self esteem into a dark place that has only sprung back into action this month with external mentorship.

Figure 2.2 Example of a copyright page. 

Chapter 4.  Learn how you write 

4.1) Everyone writes incredibly differently. 

As stated an endeavor approached from the heart, such as writing a dissertation, will inevitably be emotional, at least I have yet to meet an individual who experienced no negative emotion throughout their PhD, in fact I’ve even heard quotes like “If you haven’t considered quitting once,  you might not really be cut out for research” on the other hand, many have phrased things such as “If you love it you won’t ever want to quit”. While I can provide my personal perspective on how I operate, this will be a hurdle for nearly every individual who has written a PhD dissertation. The amount of time, care, energy, excitement, despair, hopelessness, and joy is what makes research so appealing and worthwhile for me. Upon reflection, the exact same characteristics that make one a good scientist may also be the same characteristics which impede success and growth. 

4.2) find out where and when you write the best 

This writing process was made much easier when I found time to consider why I would feel down or not able to proceed or confused by feedback and instructions, thus I suggest reflecting in a separate document or notebook (for me handwriting has been helpful in this reflection), and writing what you have done down, including the feelings or negative thoughts alongside to see if they make sense. Write down all of the joys and good things too, (Darwin has plenty of notes like this in his lab notebook published as what is now theory of evolution as described in the NPR podcast episode linked here: https://www.npr.org/sections/krulwich/2012/10/18/163181524/charles-darwin-and-the-terrible-horrible-no-good-very-bad-day.

I personally keep a notebook of every time someone has really left an impression on me in terms of encouragement, I often return to this book of documented moments in the hardest of times and reading the reality truly does help one remember that this is simply a process not an indictment or judgment of your ability or value. Additionally it serves as a sanity check to remember you do indeed belong here. Take deserve out of the grad school dictionary until merit is actually the determining factor for success. 

4.3) giving yourself and others grace & accepting/giving feedback. 

To those who ever suffer deeply from this aspect of research, the personal aspect which academia both fortunately and unfortunately inherently is paradoxical to professionalism, do not feel as though emotionality and deep care for your work and ideas is incompatible with pursuing scientific inquiry at the PhD level. One personal thing that I learned that has helped me infinitely in the past few days (i.e. the few days of me finishing up the writing process) is to think about how you critique yourself in your words, and how this may impact how you communicate with others. 

My realization or “clicking” moment was the moment I realized that part of my personal hurdles were that I assumed that I critiqued my own work in a kinder way than I do, and that this has deeply impacted my perception of both received and given feedback, in addition to causing unnecessary stress on top of what is already an incredibly stressful process. This was pointed out to me by another, and I would not have realized it on my own. 

Writing groups, or finding someone to write with can help make the process feel collaborative and fun (remember when it was fun?). Many will discuss how the PhD process caused emotional and psychological damage, and like everything in life that is known as a large undertaking, it definitely can.  However, just as the characteristics that make one a valued member of the scientific community can be the same that bar one from providing value to their fullest extent, this too can be reversed, as the process of receiving feedback and providing feedback has led me to a better understanding of my own way of processing emotions about work and life goals.
I feel as though in this regard, I have healed immensely on a psychological/cognitive level through the grace provided to me and my willingness to accept it without ego (or my attempt to). Despite potentially being unprofessional at moments due to emotionality and care for what we do as students of pathobiology, this emotional aspect is something that I believe, with time, care, and practice of acceptance of differences and encouragement of curiosity and inclusiveness, equity and fairness is achieved in a manner that is quite organic and meaningful throughout a career span. 

When discussing with other recent PhD graduates, one of the questions I ask is “What do you feel like you benefited from the most” or “What is it like on the other side do you feel different?” and overwhelmingly almost every recent graduate has told me the following in some form “I do not feel like I have to prove myself to take myself seriously anymore, I feel like I am respected and I respect others, I have grown as a person due to the confidence I had to find when I felt hopeless, and now know I can survive anything despite the hell at the end” 

Hell is the word consistent among every student I have spoken to, but much like the end of a marathon, it seems this hell is more of a backdrop to their perception of themselves before and after finishing. Amongst the older PIs, I have spoken to over my time in academia,  I have learned that the aspects which most students strive for (mostly awards and acknowledgements) are not even fully remembered, but rather the moment of insight about their project during their PhD or postdoc years are the experiences most worthwhile to discuss. I can think of two instances in which I have congratulated a PI for an extremely highly lauded award received, only to be met with confusion as if they could not remember receiving it and instead were much more excited to discuss with me their favorite textbooks from their time in research or their new whiteboard they just purchased to write down new ideas. 

Maybe it is hell-ish, but maybe it’s also part of what we notice about one another in the extremely small and thus interactive ecosystems we work in together, and providing grace to oneself and others, could provide examples of those who one can emulate to achieve success.  There is a middle ground one can find in academia for success, while everyone’s emotional processes will be different, knowing you are not alone and working together in communities resolves much of the inner critic and feelings of self deprecation as you will find, no one finds this process to be easy, and the things that can hurt the most may actually not be so relevant with respect to your future self’s memory of the time spent learning. 

Chapter 5: software & other notes. 

Everyone should be able to access and learn basic software components applicable to writing the thesis.  When in need: increase your google speed and look up overleaf.com and templates.  Here you will find templates by searching: overleaf dissertation template. Overleaf is a platform that is incredibly easy (albeit annoyingly time consuming to use) . It is a good idea to get used to this though as this is required for most journals. If I could ask for anything in terms of what would have made this process smoother it is by far this. Knowing what a thesis should look like, but not just from looking at others’ but rather looking at others’ in the context of the structure provided. It will save you so much time down the line. 

5.1 Front End Aesthetic Syntax

OVERLEAF

Overleaf uses the coding language latex, (math ppl call it lay-tech whereas biomed ppl call it Lay-tex. Weird but it’s coding so what can you do?) Software such as overleaf is based on Latex, which is essentially a language with syntax meant for producing and displaying mathematical equations, producing a consistent layout, basically anything that in the software engineering startup world would be considered “Front end”, or the aesthetic syntax if you will. 

While coding may seem intimidating, do NOT use R first. Find the easiest platform for you to use. 

Folks should understand the process that goes into these patterns for coding, people like to make it out that it’s super difficult because of the jargon, look up ANOVA proof to understand that the math is condensed by the code itself. No one is performing this by hand:.

Therefore,  My suggestion for learning basic statistical analysis required for contemporary biomedical research based in code language is the following softwares listed. 

5.2 CURRENTLY ACCEPTED SOFTWARE FOR BIOMEDICAL STATISTICS AND VISUALIZATION 

 PRISM

Basic stats better excel, for biomedical stats at least, will provide biostatistics and information about how to use them, easy interface, and helps to teach statistical analysis currently accepted in biomedical sciences, whether or not these statistics are always appropriate is beyond the scope of this document but I am open to discussing. 

MATLAB

MatLAB is the same as other languages in pattern but it’s platform is specifically not open source, and that’s for a reason. It’s incredibly powerful, the code is often already written in chunks. They have teams of people on staff who will answer questions. Many people will make fun of Matlab for being private, and I guess that makes sense, but in the end don’t let that deter you from using your free version from school! It is an invaluable resource to learning

PYTHON. 

All code is somewhat written similarly to python. It seems that python users and R users are split across the field. If you feel strongly about this then pick what works. 

MICROSOFT TEAMS

It sends automated notifications to take breaks, give yourself joy, breathe(literally), remind me to sleep or rest or stress or even notice I'm thirsty. I get into time blindness when I'm focused and to be completely honest I've never been super pain (bodily) sensitive, no clue why but I just don't realize when I'm even feeling my leg fall asleep for hours, this app is great for someone like me! I don't care how silly I look, it's good to breathe.

A larger benefit of teams is that everything stays in one place and everyone can easily share, slack is a wonderful tool but has imposed 90 day limits to messages relatively arbitrarily without warning (I am sure many of you may have experienced how devastating this was for some folks) Teams, while seemingly “outdated” I have found to be incredibly useful for sorting out my own thoughts and having some sort of defined pipeline and access to invaluable tools that would have made this process much more streamlined (the number of times I have spent hours cropping a small photo for example.

All students should have access to this mode of communication or at least a streamlined process for projects at the very beginning which is the same for all students working together or in labs which often work together even if just for convenience across servers. 

5.3 Figures & Communication:

Biorender


Helpful for graphical abstracts and very easy to use. Downside from my perspective is that now almost every figure looks the same, which can be quite limiting in research. Also, similarly to Slack, as it is not developed specifically for the school’s standards, and since it’s a newer platform still in early business stages, costs will change and reliability of its functionality may reduce over time. While a great learning tool or tool for quickly making a stunning figure, I have found it most helpful for learning and graphically drawing out what I would like to write. I typically make figures of what I will write about prior to writing as the words form while I’m creating the figure. I am certain this is not the same for everyone but it is worth considering as  a learning modality which is also time efficient. 

Adobe illustrator


If you have access, a fast and expensive computer, and time to learn, I have no doubt Illustrator will increase your confidence and add a very crucial skill to your resume. I have enjoyed learning how to make a pair of scissors and what looks like a book layout for newsletters, however, since I had no fast computation access, it often ended up taking up more time than it was worth, despite having access to the software. 

Powerpoint

 By the end of my thesis writing PowerPoint became super helpful for me because it reduced options (all the templates and predesigned cell picture from birender can make me lose the forest from the trees) Found myself making abstract figures or graphics more original which also helped me learn given the restraints in PowerPoint.  

Excel- Excel hates me but I am learning to love it

Oh excel, you and I have had our ups and downs, but in the end you make me happy because nearly everyone else can use you. Excel should be used to maintain data integrity in my opinion. There are SO many functions, and programming languages such as SAS which can be referred to for excel stats and graphical creation. Towards the end of my thesis, I actually used excel a lot for modeling abstract ideas with simple graphs that now look a lot like the figures in the philosophy of science books I admire so much and have attempted to emulate. 

5.4 Citation Managers

Citation managers work if that’s what you like to use. I necer used one well beyond papers for classes; this is due to the way I have condensed writing and literature review into one. Several that are popular are: EndNote, Mendeley, Zotero and I’m certain countless others. EndNote seems a bit ridiculous to me at this point, Mendeley is great but didn’t fit with the needs of my dissertation for whatever reason, and Zotero is somewhat just unintuitive for me. I sincerely don’t think any of these outrank doing it by hand. 

My advice? Make a google doc, pick a format from the following, and put it in a google doc that is backed up on multiple accounts and/or computers if you can somehow afford all of this. 

APA (American psychological association)

MLA (Modern Language Association)  

AMA (American Medical Association)

Or find out more here: https://pitt.libguides.com/citationhelp


5.5  Cheat codes for formatting:

To apply superscript formatting in Microsoft Word using a keyboard shortcut, you can press Ctrl, Shift, and the Plus sign (+) simultaneously:

Select the text you want to format

Press Ctrl, Shift, and + (at the same time give superscript)

To turn off superscript formatting, press the same shortcut again

Save the document to keep the new formatting

To apply subscript formatting in Microsoft Word using a keyboard shortcut, you can press Ctrl, Plus sign (+) simultaneously:

Insert section break by going to the “insert” tab at the top of google docs and scroll to break. There are section breaks and page breaks. You can also turn on “non printing characters to be able to actually read these breaks and where they are this will also pull up a paragraph symbol (no idea what that’s called). To view these go to “view” and select non printing characters.  

5.6: Artificial Intelligence, LLM( large language models), Machine Learning.

CAN YOU DEFINE THESE BEFORE USE?

Grammarly. (2024). Grammarly (May 26 version) [Large language model].

https://app.grammarly.com/ Note: Copy this citation and paste it into your document.Grammarly. (2024). Grammarly (May 26 version) [Large language model].

https://app.grammarly.com/ Note: Copy this citation and paste it into your document.

I thought this would maybe work for citations and being able to organize in docs it was not helpful. I couldn't tell what it even did-don't try the tool again. I do like it enough for when I have a big idea and I can't type it out fast enough and the predictive language helps a bit there almost like writing cursive or bullet points, but I usually return back to it and change it. 

I've noticed it makes a lot of grammar corrections that are invalid /correct literature itself which seems like it's not a great tool to do grammar checks under the current grammar used by scientists/in general. I feel it makes sentences lose all personality. Lately, I've had trouble distinguishing between emails sent in sincerity and ai crafted ones, as someone who operates how I do this is extremely hard to parse and puts me in a weird state of mind.

 Also it worries me that if everyone starts to use the same exact words, as these LLMs learn off of what has already been written and is prone to confirmation bias, it sort of feels like it's selectively restricting science based on quorum sensing, except the quorum isn't exactly giving informed consent partially because we communicate through words not chemicals, at least not consciously, I appreciate when  it corrects my spaces, I also like when it tells me I've done a good job!  Lastly it provides stats on writing (number of words used, unique words used, hours spent writing) and this helps me remind myself I did actually do work, Sometimes i'm so anxious that even if I work all day I forget, and literally rewrite the same thing (I’ve done this multiple times for school assignments like essays for Patho 1 for example). I Find it exceptionally helpful with emails and tone in a sad way because of the whole homogenous thing with language and expression.


SUPER DOWNSIDE TECHNICALLY

Even if you don’t agree that LLMs like chatGPT, alpha3, gemini, smarter child, whatever you name it, if it’s aggregating from various places, i.e. other human’s work, (think of it like wikipedia, except you just don’t want to go to wikipedia because you’d then have to cite wikipedia, which is absolutely unacceptable for most people), it is still only able to process short chunks of data (i.e your words you type) so fast. Soon, it pretty clear that these generators of aggregation will likely slow to a point that they will again become irrelevant for any sort of original thought or personal perspective bringing creative value.

Be honest with yourself for a second and think about how aggregated knowledge is neither unique to you nor created by you, as it will have more aggregated data to parse and learn from (that’s what the “learn” in these machine learning models means, really it’s training). Thus, as more people use it and add it to their platforms, exponentially more amounts of data will be algorithmically parsed, using an intensive and extreme amount of energy that will not be able to keep up with changes you’ve made to the document. 

As for the utility of these types of machine learning algorithms with a platform offer for the way I write, I found it only helpful in the context of very short portions of a document as with 20 pages of niche materials the algorithm literally takes longer to catch up with spelling and grammar errors that I do to read and correct them by hand and will cause your document to crash. 

Dissertation Citation (7 years of labor)

Howells, Jacqueline M., "Elucidating mechanisms of murine neutrophil progenitor engraftment in bone marrow" (2024). Pathobiology Theses and Dissertations. Brown Digital Repository. Brown University Library. https://repository.library.brown.edu/studio/item/bdr:49eu8uxw/

Additionally, if the above link does not work I will be happy to provide a PDF copy for anyone interested in learning more about my Dissertation Research.

Forgetting to remember: a lay summary of

This is an assignment I had in a class in the fall of 2018 in a neuro department! The directive was to provide a lay summary of a scientific article that would be read by several anonymous people (professors and non-scientists!). I’m so happy that I took so many of these writing assignments sincerely, irrespective of potential improvements I think I would make in 2024( 5ish years later).
I found it and honestly thought it was kind of neat and shared it with a few neuro focused friends who all seemed to find it interesting!

Since I received Quite encouraging sentiments that persuaded me to share this piece. Note

Please read this as what it is: a HOMEWORK ASSIGNMENT from my first ever neuro class ever so please take all claims under this context, none of these claims should be taken as approved scientifically by original authors as this was a literal homework piece and as such the portrayal of data and perspective are based solely on an attempt to demonstrate what I took from the article and tried to synthesize for a general audience, this post is meant to demonstrate a method or style of summarization of science, not science itself.



Lay summary of

Isaac Cervantes-Sandoval, Molee Chakraborty, Courtney MacMullen, Ronald L. Davis. Scribble Scaffolds a Signalosome for Active Forgetting. Neuron, 2016; DOI: 10.1016/j.neuron.2016.05.010 (1)

Forgetting to remember

Fall 2018 lay summary course assignment

Brian Eno, an innovative musician, said, “I’m always trying to do something new; I make a point of constantly trying to forget and get things out of my mind.” To value forgetfulness seems counterintuitive. We’ve all had frustrating experiences where we forget someone’s name or are unable to find our keys. Yet, recent research demonstrates that forgetting plays a vital role in learning, decision making, and mental health.

To those of us who’ve experienced the irritation and embarrassment of forgetting a birthday or blanking on account passwords, this notion is outright puzzling. How is it possible that forgetting is beneficial to memory and learning? Consider proteins associated with memory as librarians
and your memory as a library, with each book representing a different experience. Over the years, the library collects millions of books, some of which are read more than others, but all of which require space. Suppose a friend asks to borrow Shakespeare’s Hamlet for a class. Since you have millions of books, your library is massive. The librarians must walk down endless aisles looking through each shelf to find it. Even though the library contains Hamlet, it could take
so long to find that your friend’s class may have already finished. Moreover, at some point your library will fill up leaving no room for new books. How could you make this a functional library? One idea is to remove the books which have not been requested in years. Organizing shelvesby storing the most requested books closer the librarians’ desk increases retrieval efficiency and sorts the least used to the furthest shelves, making them easier to locate and discard.

This is the concept underlying how forgetting memories both makes room for storing new memories and for effectively retrieving important ones. In a 2016 study published in Neuron(1), researchers in Dr. Ron Davis’ group at The Scripps Research Institute explored the biological mechanisms of forgetting using Drosophila, commonly known as the fruit fly.

Flies are an excellent model for studying human memory because they are easy to work with given their quick development and short lifespan. They also have similar brain circuitry and genes related to memory to humans. Flies have a relatively simple genetic architecture, making it easier to study genes involved in behavior through DNA modification. DNA provides instructions to make proteins the same way a recipe provides instructions for making cookies.

By removing ingredients one at a time, it’s possible to determine the ingredients important for the cookie’s taste and consistency. Likewise, editing DNA is a useful tool for deducing which genes are important for a given trait. In this study, researchers investigate the role of the gene Scribble in the process of forgetting. In both humans and flies, memory of smell is key for survival. Smells can indicate if food is safe to eat based on previous experiences.

Memory of smell is unique among the senses because the region of the brain involved with odor perception communicates directly with areas important for memory and emotion. A rotten odor evokes feelings of illness that causes avoidance of harmful substances.

In this study, researchers exposed flies to an odor while simultaneously shocking the flies with electricity. During this “learning session,” normal flies learn that the odor is associated with pain. When exposed to the odor again during a “testing session”, flies that remember the shock avoid the odor while those who have forgotten do not. The authors investigated genes involved in forgetting by deleting thousands of different genes and performing this odor memory experiment.

Normally flies forget odor associated shock a few hours after the learning session. When researchers deleted a gene called Scribble, flies avoided the shock associated odor during testing sessions 24 hours after the learning session, long after the normal flies have forgotten. This indicates that Scribble is required to forget. While retaining memories to avoid future pain seems great, researchers sought to understand if memory retention interferes with memory formation.

To investigate this, they again exposed flies to an odor and shock. After enough time passed for normal flies to forget, they exposed flies to a new odor along with shock. In the following testing session, normal flies ran away from the new odor source while Scribbleless flies did not. Researchers hypothesize that Scribbleless flies’ retention of old memories inhibits new memory formation. Like a library with completely packed shelves, there is no space for new material unless books are removed.

After demonstrating that Scribble is involved with forgetting, researches then explored how the process actually works. The Scribble gene encodes a scaffolding protein that provides a framework supporting other proteins. Using the library analogy, Scribble can be compared to a rolling ladder that other proteins use to carry out librarian duties. The authors found that Scribble binds to Rac, another protein essential for forgetting memories.

Rac accomplishes this by remodelling cellular structure. When a neuron receives a signal to forget, Rac uses the Scribble “ladder” to reach books and discard them. Scribble provides a structure that allows Rac to erase
memories creating space for new ones. This research provides new insights into the mechanisms underlying the process of forgetting.

Dr. Ron Davis elaborated on the significance of this study’s findings in an interview with Science Daily,“Understanding the process of forgetting could have an enormous impact on how we treat a whole range of diseases. Certain memories are intrusive and, with sufficient knowledge of how the brain forgets, we should be able to remove selective memories.” (2). This study provides foundation for future research on the process of forgetting and its effect on learning and memory.


References
1. Isaac Cervantes-Sandoval, Molee Chakraborty, Courtney MacMullen, Ronald L. Davis. Scribble Scaffolds a Signalosome for Active Forgetting. Neuron, 2016; DOI: 10.1016/j.neuron.2016.05.010


2. Scripps Research Institute. "Scientists discover new protein crucial to normal forgetting.” ScienceDaily. ScienceDaily, 2 June 2016.
www.sciencedaily.com/releases/2016/06/160602132422.html

Part 2 finishing up


https://vimeo.com/943769479

Click the link to hear a 6 minute update from a scientist finishing up a PhD! My goal was mostly to feel I was being true to myself and sometimes I hear myself say something and it’s cathartic. Moreover, I typically find it’s important to express what tedious parts emotionally rest under the wondrous moment of being “done” and in part I think am describing the anxieties of entering a world uncertainm

https://vimeo.com/943769479

Audiophiles and philosophiles: here are some curious thoughts

——> click below Curious human reflections 1

I love podcasts and telling people about my half-formed ideas to refine them. Here. I’m excited to share a recent audio musing on the end of the era of graduate school training, how words are shared across fields, and their meaning to me as a student for life who also has a unique gift of observation derived from complex post-traumatic stress disorder. This perspective should be taken as such: mostly just curious about these topics and making statements or asking questions in an attempt, a first real attempt, at igniting and helping instead of drowning ideas and fellow humans.

Don’t mistake things that are about to bloom for no value

Oh the places you'll go!

Oh the beginning of graduate school. How naive I was! But I'm so proud of how far I have come. I entered feeling so excited to meet many of my intellectual heroes, and I learned a lot of them were in fact not truly what I expected. In fact, the greatest joy of all has been proving to myself over and over that the biggest names and powers in ivy league schools, carry what I feel is an embarrassingly unearned prestige (I am literally full of shame when folks are so impressed the moment I tell them where I go to graduate school bc of the disparity between prestige and true merit). Understanding how to discern paper prestige from real honest merit based prestige has opened my eyes to how much better of a scientist I can be if I focus on my curiousity and believe in myself. No matter how many people in my life who hold titles like "full tenured professor, director of X, endorsed by XYZ, editor of X prestigious magazine" have told me all the ways I don't deserve to pursue biomedical research. I have learned to laugh for they are so out of touch, that I never needed their approval in the first place. Despite how I have been treated and how I see countless other students being treated, I still believe in using the tools we have in the best way possible. I am an example of someone who had nearly every door slammed in my face, but somehow, through my own curiousity and responsibility to the public who has paid for my way through school and provided me the opportunity to earn a PhD, I have managed to kick down each and every one of those doors to learn and teach something new to someone. I am so grateful to have had the opportunity to see past the curtain and learn how to recognize scientists who want to help, and opportunists to avoid at all costs, but most importantly that real talent, if fostered never ever cedes. Still here, and still excited.

Reading Biography

Recently, I was interviewed by a high school student for an assignment to write a “reading biography” of an adult. The questions were broken up into three sections: Before you knew how to read, learning how to read/childhood reading patterns, and finally what place reading holds in your adult life. I really enjoyed answering these questions. The past two years have been a process of extreme growth for me, and like most growth, there is inherently loss. I am grateful for being asked to help in this assignment, as it helped me really understand what reading is to me and how it has influenced my life. Below you will find the interview questions and my answers. Would love to see other people’s feelings on these questions!

Early childhood memories: Prior to learning  how to read

  1. What place did books and reading have in your life as a very young child? 

    • Everything. I remember having a few books of my own and my mother reading them to me. 

  2. Did you see your parents or family members read?

    • Yes I saw my mom reading all the time. 

  3. Do you remember what you first read? 

    • I remember reading the monster at the end of this book, which is a story about Grover from sesame street who is terrified of a monster, and at the end he finds out the monster was him and he didn’t have anything to really fear. 

  4. Did you have any of your own books? What was your favorite? 

    • I know that I owned goldilocks and the three bears and that was one of my favorites.

  5. Did you pretend to “read” books to yourself or others? 

    • Yes apparently so, my mom told me that when she would read me stories I would pretend to read, although she seems to think I was actually reading, which may or may not have been the case. I don’t remember a time in my life when I couldn't read. 

  6. Did anyone take you to the library or the bookstore? 

    • Yes. My mom took me to the library whenever I could go. It was the greatest experience. West Portal mostly, and the public library in the Richmond district as well as San Francisco’s main library at Civic Center which is one of my most beloved places in the whole world. 

Childhood memories: Learning  how to read

Childhood Memories of Learning to Read

  1. When you first learned how to read what was that like for you?

    • I really don’t remember a time in my life when I couldn’t read, but reading has always been and will always be a gorgeous experience for me. 

  2. When, where and how did you learn?

    • I honestly think I learned from making my mom read me books as a very young child and just picking up on the symbols. I never felt like I “learned” it in the traditional sense. Rather, after seeing similar patterns over and over, it became intuitive, like knowing yellow is yellow without having to practice seeing yellow. 

  3. Did someone teach you or did you teach yourself?

    • I don’t think it’s possible to teach yourself how to read because the author, a parent reading the words to you, is necessary for linking the letter symbols to actual phenomena or objects. Imagine learning how to read if no other human existed on the planet? You would not have materials to learn context from. It’s a bit like John Searle’s Chinese room thought experiment: 

      1. Searle's thought experiment begins with this hypothetical premise: suppose that artificial intelligence research has succeeded in constructing a computer that behaves as if it understands Chinese. It takes Chinese characters as input and, by following the instructions of a computer program, produces other Chinese characters, which it presents as output. Suppose, says Searle, that this computer performs its task so convincingly that it comfortably passes the Turing test: it convinces a human Chinese speaker that the program is itself a live Chinese speaker. To all of the questions that the person asks, it makes appropriate responses, such that any Chinese speaker would be convinced that they are talking to another Chinese-speaking human being.

      2. The question Searle wants to answer is this: does the machine literally "understand" Chinese? Or is it merely simulating the ability to understand Chinese?

      3. Source: wikipedia (click link to get to the page describing this thought experiment and where the quote from above comes from. 

  4. Do you remember what you first read? 

    • No, I think it was likely The Monster at the End of this Book or The Goldilocks book. 

  5. How did this feel for you to be able to read?

    • I cannot imagine not being able to, so I assume I feel very happy. 

  6. Did you have your own books? 

    • Yes, at least the two I mentioned. 

  7. Did you get your own library card?

    • Yes, and I used it often. I also find libraries to be wonderful places to get books that have notations in them. There’s something comforting about reading a strangers notes in a book, or what they underlined as it spoke to them in some way. It feels like traveling through space and time and connecting with the author, this person, and yourself. 

  8. Did you get to choose your own books? 

    • Yes always. 

School Memories (reading in elementary school) 

  1. Did you  learn how to read in elementary school or did you already know how to read? 

    • Yes I think so, I don’t remember not being able to. 

  2. Was reading a pleasure or a chore

    • I dislike this binary of pleasure v chore. Reading for me has always been and will always be an intrinsic  part of my life and who I am. Reading feels the same as breathing, which is neither pleasure nor chore at times, while at other times can be either or. 

  3. Were you given time each day to read whatever you wanted?

    • I think so. Or at least I forced the time even if I had to stay up all night. 

  4. Did you have favorite places you’d like to read?

    • Yes, I liked to read everywhere but especially by the window in my room. When I was incredibly immersed in a book it didn’t matter where I was or how I was feeling though. I read while walking, eating, and bathing, definitely almost hurt myself a couple of times due to how immersed I was.  

  5. Did you ever read things that you didn’t have to? 

    • Yes all the time. 

  6. Did you readcomics/manga/graphic novels? 

    • I read a couple I found at the library.

  7. Which was your favorite 

    • I  love X-men 

  8. What was it about?

    • X-men comics are about a group of individuals who are ostracized from society due to superhuman powers (that tend to not be all that superhuman at all in the end, as this is a double-edged sword) who all come together to find peace from their isolation and banishment. They are all at a school for people with these abilities who are different and work together to try to do what they can to help society and each other. They are all flawed, and many have had to crawl through the saddest of circumstances to find a group they belong to, they continue to make mistakes, but always try to understand each other and help one another out. 

  9. Did you subscribe to any magazines?

    • No, but I do now! 

  10. What did you think of the school library? 

    • I really loved it. It was peaceful. 

  11. Did you ever read at night with a flashlight

    • All the time. 

  12. Did you ever steal a book? 

    • Not to my knowledge haha. I might have forgotten to return a library book before though. 




Adolescence and reading 

  1. Period of time for finding oneself during this time in your life did you have a favorite book or author? 

    • The Sound and the Fury by William Faulkner was my favorite book at the time, and my favorite author was probably Kurt Vonnegut after reading Cat's cradle. 

  2. How did this book or writer affect you? 

    • The Sound and The Fury was a powerful force in my life at that time. I had no idea that books could be laid out in such a complex manner, from multiple perspectives. It was the first time a book felt much more like a puzzle, where I had to really put myself in the character's shoes and walk a mile in them in order to find the key to unlock the plot. I could see for the first time how different people perceive events, and how a group of people can fall apart due to these differences. The book title is actually derived from a line in Shakespeare’s Macbeth “ Out, out, brief candle. Told by an idiot, full of sound and fury, Signifying nothing.” I feel this quote is more relevant today than ever. 

    • I cannot emphasize the profound impact of Kurt Vonnegut on my life. I have read all of his books, and watched scores of interviews. Every time I read Cat’s Cradle, (which has been in the most difficult moments of my life) I feel such a sense of understanding. Such a sense of the importance of humility and kindness that I can only strive to achieve. 

  3. Do you still have a copy of this book

    • I checked out the sound and the fury from the library so I don’t have a copy and I’m not sure I need one. It feels like it would be really hard to go back to re-read that book due to its darkness and how much I related to certain scenarios and characters. 

    • I still have Cat's Cradle, although I lost my original and had to buy a new copy. 

  4. At some difficult time in your life did someone encourage you to keep reading? 

    • Ms. Fuller at Lowell. She encouraged me to pursue graduate studies in literature when I was in high school. We were reading Catcher in the Rye by J.D. Salinger and at this point in my life, I was really struggling with depression and anxiety. We were discussing a portion of the book where at the end Holden Caulfield, the story’s jaded protagonist, gets jolted out of his depression and resentment when he sees his younger sister playing in a golden field of rye. It was a moment in time. I raised my hand during the discussion to say how I understood this feeling. I related to Holden for the first time at this point in the plot because it felt as though I had experienced the same quiet revolution in my emotions when I saw my little brother jumping with a huge smile in a jumpy house for a birthday party.  He seemed so full of joy and so free that tears started to just fall from my eyes, because at that moment, I felt alive too. I felt like those are the moments that we hang on to in times of suffering. They are simple moments. They cannot be bought or sold, they can’t even really be found unless that quiet revolutionary realization has occurred:  that life is full of pain but also full of these beautiful moments of joy, and to open your eyes to the joy, if only for a moment in time. 

  5. Was there a point in your life where you realized you could read anything?

    • I felt I could read whatever I wanted my whole life, it didn’t feel like a choice or an ability, it felt like a necessity. I would read anything I could get my hands on ever since I can remember. 

  6. Did reading ever get you in trouble? 

    • I have gotten in trouble for the specific act of reading so much or re-reading the same books in high school that I would switch out the covers so that I could read whatever I wanted to avoid punishment for pleasure reading or compulsive reading. Depending on how you define trouble and what it means to read versus comprehend. One can read words but lack the ability to comprehend what the meaning is. Comprehension allows for interpretation and subsequent connection to other concepts and experiences. Reading has gotten me in tons of trouble indirectly in this manner. The more that you read, the more that you know. While knowledge is powerful, it can also be a heavy burden to bear as it changes your perspective on things. As they say ignorance is bliss, and comprehending what one is reading has troubled me internally in this way. 

  7. Did anyone ever censure your materials?

    • Not really, no. I would argue that most teachers I had up until graduate school now always encouraged me to read censored books and for that I am grateful as those books carry much knowledge about the world and how it operates. 

  8. Did you ever hide you reading materials, if so why? 

    • I hid books my mom would get mad at me for reading over and over again. But that’s about it. 

  9. When did you become aware that certain books were banned?

    • Sometime in elementary school, we had a banned books day. I didn’t get it.

Adult Memories of Reading

  1. What place does reading have in your adult life?

    •  Most of what I do is reading. As a PhD student studying pathobiology, it is required to at least read some high level technical work and to firmly comprehend the meaning and concepts of these works. Many people in the sciences do not like reading much, and would prefer to perform experiments most of the time without reading a lot to understand what the implications of those experiments are, but learn after the fact. I prefer to read as much as I can, because I do not feel comfortable doing experiments without grasping why and thoroughly understanding how. Further reading is what I have dedicated the majority (I am talking 90%) of my time outside of sleep to, sometimes forgoing sleep to read. I especially feel comfort in reading academic papers outside of my field to understand how my work fits in with the collective human knowledge base. I have learned that only reading vast arrays of opinions, ideas, concepts, etc is the only way to make sense of the world for me.  Reading is like a dear old friend that never fails to help me in some way.

  2. When you read for pleasure what do you read?

    • I used to read mostly novels and fiction. Since the pandemic started many of the fiction books I  have tried to pick up to read have felt very sad or very happy and thus have been difficult to relate to. When things are very sad it’s hard for me not to feel that sadness myself, and I don’t have space for more sadness, particularly for fictional characters. When the fiction is very happy, it is again difficult for me to relate as it feels almost gauche compared to what we have all collectively experienced.  For pleasure, I now read a lot of philosophy books and poetry. I suspect this is due to their abstract nature which provides reprieve for me. 

    • I also read manga as I have gotten very into anime. I like Berserk (not for children!), Monster, Jujitsu Kaisen, Akira, Gundam, Pun Pun (not for children) 

  3. What do you wish you spent  more time reading? 

    • Nothing, I read  whatever I want whenever I want to. 

  4. Do you have a “to read stacks” of books

    • Yes, it’s more like a stack of books I read at the same time returning to an older one I haven't finished if I get overwhelmed or bored of the currently read one. 

  5. Do you buy books for yourself or others? 

    • Always. It’s my favorite kind of gift to give to people. There’s nothing more wonderful than receiving a book or reading materials, I love walking around in book stores thinking about what books would suit which person, and often I find myself doing this regularly.

  6. Are there books that you give to others in times of crisis

    • Yes, depending on the person there are two books. One is Tiny beautiful things by Cheryl Strayed which is a collection of tiny beautiful accounts. I also always recommend Cat’s Cradle by Kurt Vonnegut as it depicts the absurdities of life in a very serious but also digestible fashion. “Tiger got to eat, bird got to fly, man gotta sit around and wonder why why why, Tiger got to eat, bird got to land, man got to tell himself he understands”' is my favorite line that I painted on my door in college and say to myself  pretty much daily.  

    • Recently I have been resonating a lot with this quote from James Baldwin which I think is helpful in times of crisis:

       “The Artist’s Struggle for Integrity” 

      “I really don’t like words like “artist” or “integrity” or “courage” or “nobility.” I have a kind of distrust of all those words because I don’t really know what they mean, any more than I really know what such words as “democracy” or “peace” or “peace-loving” or “warlike” or “integration” mean. And yet one is compelled to recognize that all these imprecise words are attempts made by us all to get to something which is real and which lives behind the words. Whether I like it or not, for example, and no matter what I call myself, I suppose the only word for me, when the chips are down, is that I am an artist. There is such a thing. There is such a thing as integrity. Some people are noble. There is such a thing as courage. The terrible thing is that the reality behind these words depends ultimately on what the human being (meaning every single one of us) believes to be real. The terrible thing is that the reality behind all these words depends on choices one has got to make, for ever and ever and ever, every day. I  am not interested really in talking to you as an artist. It seems to me that the artist’s struggle for his integrity must be considered as a kind of metaphor for the struggle, which is universal and daily, of all human beings on the face of this globe to get to become human beings. It is not your fault, it is not my fault, that I write. And I never would come before you in the position of a complainant for doing something that I must do… The poets (by which I mean all artists) are finally the only people who know the truth about us. Soldiers don’t. Statesmen don’t. Priests don’t. Union leaders don’t. Only poets.[This is] a time … when something awful is happening to a civilization, when it ceases to produce poets, and, what is even more crucial, when it ceases in any way whatever to believe in the report that only the poets can make. Conrad told us a long time ago…: “Woe to that man who does not put his trust in life.” Henry James said, “Live, live all you can. It’s a mistake not to.” And Shakespeare said — and this is what I take to be the truth about everybody’s life all of the time — “Out of this nettle, danger, we pluck this flower, safety.” Art is here to prove, and to help one bear, the fact that all safety is an illusion. In this sense, all artists are divorced from and even necessarily opposed to any system whatever”

  7. Tell of one particular book or author and explain its impact on your adult life. 

    • The Jakarta Method- a nonfiction account of what happened to Indonesia after the second world war.  This is where I believe sometimes reading can be a double edged sword. When I learned the historical aspects delineated in this book, it completely changed the way I see the world. But I was in pain the entire time reading it, as I desperately wanted it to be fiction. 

    • The second is a poem by Charles Bukowski “The Crunch” which helped me feel less alone in the confusing thoughts I had these past few years as I have watched in shock people do such cruel things with little remorse, even our society as a whole allows for this cruelty. It helped me see myself and not feel immense fear about why people behave the way they do. I suppose one could say it helped me to mature and to be less naive about the human condition. That there is beauty, in an untouched person, lonely, watering a plant. 

    The Crunch

    Love is a Dog From Hell - 1977

    ---

    too much

    too little

    too fat

    too thin

    or nobody.

    laughter or

    tears

    haters

    lovers

    strangers with faces like

    the backs of

    thumb tacks

    armies running through

    streets of blood

    waving winebottles

    bayoneting

    an old guy in a cheap room

    with a photograph of M. Monroe.

    there is a loneliness in this world so great

    that you can see it in the slow movement of

    the hands of a clock

    people so tired

    mutilated

    either by love or no love.

    people just are not good to each other

    one on one.

    the rich are not good to the rich

    the poor are not good to the poor.

    we are afraid.

    our educational system tells us

    that we can all be

    big-ass winners.

    it hasn't told us

    about the gutters

    or the suicides.

    or the terror of one person

    aching in one place

    alone

    untouched

    unspoken to

    watering a plant.

    people are not good to each other.

    people are not good to each other.

    people are not good to each other.

    I suppose they never will be.

    I don't ask them to be.

    but sometimes I think about

    it.

    the beads will swing

    the clouds will cloud

    and the killer will behead the child

    like taking a bite out of an ice cream cone.

    too much

    too little

    too fat

    too thin

    or nobody

    more haters than lovers.

    people are not good to each other.

    perhaps if they were

    our deaths would not be so sad.

    meanwhile I look at young girls

    stems

    flowers of chance.

    there must be a way.

    surely there must be a way that we have not yet

    though of.

    who put this brain inside of me?

    it cries

    it demands

    it says that there is a chance.

    it will not say

    "no."





No one is coming to help you. Let’s help each other.

In the past few year I have learned so much about what community means to me. When I started my PhD journey, I was so incredibly excited to be able to learn how to ask interesting questions that ultimately would reduce human suffering. I started out as a trained evolutionary biologist and ecologist, which perhaps lends me this perspective of community being vital to any individual success or failure. No particular community structure fits all sizes, but I have seen the reality of ecological impacts on geologically rapid evolution in lizards in White Sands, and I know humans can “co-evolve” to to work within the bounds of their ecosystem.

I believed that the bounds of the ecosystem, i.e. the biotic and abiotic factors of graduate school boiled down to hard work, sincere effort, a dash of talent and luck. I was sorely mistaken. This was an individualist outlook, but one that was easy to exploit.

Now, after many years, and many iterations of trying to find the resources available to me as a graduate student, I’ve learned how important it is to know how to maneuver through a world of politics, cruelty, viciousness and bitterness that has entrenched some of the brightest minds in science. I have learned title and status are really meaningless unless they have actions which back them up.

I have learned that my peers are the next generation of scientists who have a responsibility to the world based on our privileges provided by academia, the status, the relative security, the ability to act without consequence as you “level up”. I have learned that I cannot determine what that responsibility is without engaging with people both in and outside of academia to discuss value systems. Science has failed us in this period of crisis.

No one is coming to help you. Let’s help each other.

Just some musings on neuroimmune signaling in health and disease

Please click link here: BINS Neuroimmunology Discussion Presentation

I was lucky to give to a group of interested interdisciplinary sciences . I wanted to share this because I think neuro-immune signaling is one of the most fascinating fields and likely key for understanding behavior and aging. I find that the fact that aging is not linear in terms of healthspan, it needs an incredibly flexible system in order to maintain it (the immune system, but take that with a grain of salt as I’m an immunologist and biased :) ). One of my favorite questions I was asked during this discussion was "Are you saying that Alzheimer’s is transmissible?” . My favorite comment was about how we use the word “memory” to describe both cognitive and immunological systems: “Words have to mean something!”

I would love more and more of these interdisciplinary conversations like this, this is how I see the future of science. To do so we have to explain our jargon and that requires looking at the field from a more philosophical position (at least in my opinion!), where people are open to new ideas and collaborative thought. Where it’s assumed people have an interest but may not have the knowledge (it’s impossible to know everything despite us all pretending we do.

I’ve also been quite interested for some time in the relationship of C.albicans and neurodegeneration reading the work of Dr. Moir and Dr. Tanzi. Moreover, C. albicans is a common commensal bacteria that also demonstrates incredible flexibility to it’s environment (as is an aging human body). I also have been fascinated by neutrophils, for their TRAP capabilities, and Th17 cells, for no discernable reason except for their strange relationship with Th1 cells I can’t quite put my finger on yet. Anyway, enough musings for today!

Source: file:///C:/Users/14152/Downloads/BINS_Neur...

Lunch with a former PI, Dr. David Raulet

It was so great to see my former PI from Berkeley. Dr. David Raulet came to visit Brown and gave a fascinating talk on his most recent work on NK cellular therapy potentials. During Q&E shared some of his interesting thoughts on cancer from an evolutionary perspective (would love to read/understand more). Also, it was wonderful to actually understand the actual immunology much more than I did the last time I had seen him when I graduated from Berkeley in 2013 ( coincindentally in a Berkeley pizza staple: Jupiter. Here we are at a Brown University pizza staple: Flatbread) :)

Providence Area Aging Research Forum (PAARF) Presentation

Hi!

I’m super stoked to share that I will be presenting my research on how the dysregulation of the immune signaling pathways impacts neurodegeneration on a molecular and cellular level. I am so happy to be afforded this opportunity to present new and exciting work to the general public in a casual setting. Age related disease impacts so many people and I hope to be able to share what I have learned in my progress here at Brown. The other talks are sure to be amazing as well!